Protein_Domain
Part:BBa_K4181027
Designed by: Junzhi Yan Group: iGEM22_SJTU-BioX-Shanghai (2022-10-11)
Dsup- K198M_V195A
We directed the evolution of the DSUP protein to change lysine at position 198 to methionine and valine at position 195 to alanine.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 515
Illegal PstI site found at 1139 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 515
Illegal PstI site found at 1139 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 163
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 515
Illegal PstI site found at 1139 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 515
Illegal PstI site found at 1139
Illegal AgeI site found at 121 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 197
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Categories
Parameters
None |